Oxidative Stress In HIV

Total antioxidant capacity – a novel early bio-chemical marker of
oxidative stress in HIV infected individuals
Journal of Biomedical Science 2009, 16:61doi:10.1186/1423-0127-16-

61
DR Suresh1 , Vamseedhar Annam2 , K Pratibha1 and BV Maruti Prasad3
1Department of Biochemistry, Sri Siddhartha Medical College, Tumkur,
India
2Department of Pathology, Sri Siddhartha Medical College, Tumkur,
India
3Department of Biochemistry, Bangalore Medical College, Bangalore,
India


Abstract
Background
Oxidative stress induced by the production of reactive oxygen species
may play a critical role in the stimulation of HIV replication and
the
development of immunodeficiency. This study was conducted as there
are
limited and inconclusive studies on the significance of a novel early
marker of oxidative stress which can reflect the total antioxidant
capacity in HIV patients,


Methods
Total antioxidant capacity (TAC) and lipid peroxidation were
evaluated
in 50 HIV-1 seropositive patients (including HIV-1 symptomatics and
asymptomatics). Controls included 50 age and sex matched and
apparently healthy HIV-1 seronegative subjects. Serum malondialdehyde
(MDA), Total antioxidant capacity [TAC] (by ferric reducing
antioxidant power assay), vitamin E, vitamin C and superoxide
dismutase (SOD) enzyme activity were estimated among controls and
cases. Statistical comparisons and correlations at 5% level of
significance were determined.


Results and Discussion
The mean MDA concentrations were significantly elevated in both HIV-1
asymptomatic (CD4+ count > 500 cells/microliter) and HIV-1
symptomatic
(CD4+ count <500> asymptomatic >
symptomatic) compared to controls (p <> asymptomatics > controls. TAC can be used as a novel early bio-


chemical marker of oxidative stress in HIV-1 infected patients which
may result in reduced tissue damage by free radicals and help to
monitor and optimize antioxidant therapy in such patients.

This is an Open Access article distributed under the terms of the
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licenses/by/2.0), which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly
cited.
The electronic version of this article is the complete one and can be
found online at: http://www.jbiomedsci.com/content/16/1/61

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